Artisans in the North Carolina Backcountry

During the quarter of a century before the thirteen colonies became a nation, the northwest quadrant of North Carolina had just begun to attract permanent settlers. This seemingly primitive area may not appear to be a likely source for attractive pottery and ornate silverware and furniture, much less for an audience to appreciate these refinements. Yet such crafts were not confined to urban centers, and artisans, like other colonists, were striving to create better lives for themselves as well as to practice their trades. As Johanna Miller Lewis shows in this pivotal study of colonial history and material culture, the growing population of Rowan County required not only blacksmiths, saddlers, and tanners but also a great variety of skilled craftsmen to help raise the standard of living. Rowan County\u27s rapid expansion was in part the result of the planned settlements of the Moravian Church. Because the Moravians maintained careful records, historians have previously credited church artisans with greater skill and more economic awareness than non-church craftsmen. Through meticulous attention to court and private records, deeds, wills, and other sources, Lewis reveals the Moravian failure to keep up with the pace of development occurring elsewhere in the county. Challenging the traditional belief that southern backcountry life was primitive, Lewis shows that many artisans held public office and wielded power in the public sphere. She also examines women weavers and spinsters as an integral part of the population. All artisans—Moravian and non-Moravian, male and female—helped the local market economy expand to include coastal and trans-Atlantic trade. Lewis\u27s book contributes meaningfully to the debate over self-sufficiency and capitalism in rural America. Johanna Miller Lewis is assistant professor of history and assistant coordinator of the public history program at the University of Arkansas at Little Rock. Miller\u27s book provides fresh insight into the workings of consumer culture and the market economy in a region of colonial America we are beginning to understand better. —Arkansas Historical Quarterly A shrewd and insightful book that overturns previous misconceptions about the absence of artisans in the backcountry, settlers \u27self-sufficiency,\u27 and the growth of capitalism in agrarian America. —Jeffrey J. Crow Breaks new ground on several scores. —Journal of American History Lewis has broadened our perception of backcountry life by providing a great deal of useful information on a previously neglected topic. —Journal of Appalachian Studies Weds several themes in the historiography of colonial America by examining artisans on the colonial North Carolina frontier and by infusing these men and women into the emerging frontier market to investigate self-sufficiency and capitalism in rural America. —Labor Historyhttps://uknowledge.uky.edu/upk_united_states_history/1128/thumbnail.jp

Artisans in the Carolina backcountry: Rowan County, 1753-1770

Artisans played an important role in the social and economic life of Rowan County, North Carolina beginning with its creation in 1753. Whether they came individually with their families to obtain land and establish new lives, or they were chosen by the Moravian Church to settle the 100,000 acre Wachovia Tract, all of these artisans were part of the huge wave of immigration to the backcountry of North Carolina which occurred during the third quarter of the eighteenth century.;The development of the artisan population paralleled the growth of Rowan County. In the early 1750s a handful of artisans produced objects that the small groups of settlers needed to survive and create new lives in the backcountry. Blacksmiths, weavers, tailors, tanners, and saddlers made clothes, shoes, saddles, and ironware for backcountry inhabitants; and millwrights and carpenters built structures which helped Rowan County develop.;As more people poured into the county (which consisted of the northwest quadrant of the colony) so did more artisans. Hatters, joiners, masons, coopers, turners, wheelwrights, wagonmakers, potters and gunsmiths joined the expanding community of craftspeople. Simultaneously, improvements and growth in the road and ferry system increased the range of local trade networks all the way to the coast, and across the Atlantic Ocean. While backcountry residents once looked to Cross Creek, Charles Town, or London, to fill their desire for conspicuous consumption, local silversmiths, cabinetmakers, gunstockers, and watchmakers came to fill their needs. Public and private accounts record artisans making raised paneled room interiors, silver shoe buckles, fancy beaver hats, walnut tables and chests of drawers, and fancy riding chairs for a demanding clientele.;No other studies of Rowan County or the North Carolina backcountry have focused on the artisans of that region. Research in the county\u27s court records, apprentice bonds, deeds, and wills, as well as extant invoices and account books, indicates that artisans played a significant role in increasing the quality of life in backcountry North Carolina. The presence of artisans and the availability of their products in Rowan County shows that inhabitants of the backcountry did not always live in the most slovenly manner described by many historians

Pan-Cancer Analysis of lncRNA Regulation Supports Their Targeting of Cancer Genes in Each Tumor Context

Long noncoding RNAs (lncRNAs) are commonly dys-regulated in tumors, but only a handful are known toplay pathophysiological roles in cancer. We inferredlncRNAs that dysregulate cancer pathways, onco-genes, and tumor suppressors (cancer genes) bymodeling their effects on the activity of transcriptionfactors, RNA-binding proteins, and microRNAs in5,185 TCGA tumors and 1,019 ENCODE assays.Our predictions included hundreds of candidateonco- and tumor-suppressor lncRNAs (cancerlncRNAs) whose somatic alterations account for thedysregulation of dozens of cancer genes and path-ways in each of 14 tumor contexts. To demonstrateproof of concept, we showed that perturbations tar-geting OIP5-AS1 (an inferred tumor suppressor) andTUG1 and WT1-AS (inferred onco-lncRNAs) dysre-gulated cancer genes and altered proliferation ofbreast and gynecologic cancer cells. Our analysis in-dicates that, although most lncRNAs are dysregu-lated in a tumor-specific manner, some, includingOIP5-AS1, TUG1, NEAT1, MEG3, and TSIX, synergis-tically dysregulate cancer pathways in multiple tumorcontexts

Pan-cancer Alterations of the MYC Oncogene and Its Proximal Network across the Cancer Genome Atlas

Although theMYConcogene has been implicated incancer, a systematic assessment of alterations ofMYC, related transcription factors, and co-regulatoryproteins, forming the proximal MYC network (PMN),across human cancers is lacking. Using computa-tional approaches, we define genomic and proteo-mic features associated with MYC and the PMNacross the 33 cancers of The Cancer Genome Atlas.Pan-cancer, 28% of all samples had at least one ofthe MYC paralogs amplified. In contrast, the MYCantagonists MGA and MNT were the most frequentlymutated or deleted members, proposing a roleas tumor suppressors.MYCalterations were mutu-ally exclusive withPIK3CA,PTEN,APC,orBRAFalterations, suggesting that MYC is a distinct onco-genic driver. Expression analysis revealed MYC-associated pathways in tumor subtypes, such asimmune response and growth factor signaling; chro-matin, translation, and DNA replication/repair wereconserved pan-cancer. This analysis reveals insightsinto MYC biology and is a reference for biomarkersand therapeutics for cancers with alterations ofMYC or the PMN

Genomic, Pathway Network, and Immunologic Features Distinguishing Squamous Carcinomas

This integrated, multiplatform PanCancer Atlas study co-mapped and identified distinguishing molecular features of squamous cell carcinomas (SCCs) from five sites associated with smokin

Spatial Organization and Molecular Correlation of Tumor-Infiltrating Lymphocytes Using Deep Learning on Pathology Images

Beyond sample curation and basic pathologic characterization, the digitized H&E-stained images of TCGA samples remain underutilized. To highlight this resource, we present mappings of tumorinfiltrating lymphocytes (TILs) based on H&E images from 13 TCGA tumor types. These TIL maps are derived through computational staining using a convolutional neural network trained to classify patches of images. Affinity propagation revealed local spatial structure in TIL patterns and correlation with overall survival. TIL map structural patterns were grouped using standard histopathological parameters. These patterns are enriched in particular T cell subpopulations derived from molecular measures. TIL densities and spatial structure were differentially enriched among tumor types, immune subtypes, and tumor molecular subtypes, implying that spatial infiltrate state could reflect particular tumor cell aberration states. Obtaining spatial lymphocytic patterns linked to the rich genomic characterization of TCGA samples demonstrates one use for the TCGA image archives with insights into the tumor-immune microenvironment

Hormone-replacement therapy influences gene expression profiles and is associated with breast-cancer prognosis: a cohort study

BACKGROUND: Postmenopausal hormone-replacement therapy (HRT) increases breast-cancer risk. The influence of HRT on the biology of the primary tumor, however, is not well understood. METHODS: We obtained breast-cancer gene expression profiles using Affymetrix human genome U133A arrays. We examined the relationship between HRT-regulated gene profiles, tumor characteristics, and recurrence-free survival in 72 postmenopausal women. RESULTS: HRT use in patients with estrogen receptor (ER) protein positive tumors (n = 72) was associated with an altered regulation of 276 genes. Expression profiles based on these genes clustered ER-positive tumors into two molecular subclasses, one of which was associated with HRT use and had significantly better recurrence free survival despite lower ER levels. A comparison with external data suggested that gene regulation in tumors associated with HRT was negatively correlated with gene regulation induced by short-term estrogen exposure, but positively correlated with the effect of tamoxifen. CONCLUSION: Our findings suggest that post-menopausal HRT use is associated with a distinct gene expression profile related to better recurrence-free survival and lower ER protein levels. Tentatively, HRT-associated gene expression in tumors resembles the effect of tamoxifen exposure on MCF-7 cells

A Molecular Epidemiological Study of var Gene Diversity to Characterize the Reservoir of Plasmodium falciparum in Humans in Africa

BACKGROUND: The reservoir of Plasmodium infection in humans has traditionally been defined by blood slide positivity. This study was designed to characterize the local reservoir of infection in relation to the diverse var genes that encode the major surface antigen of Plasmodium falciparum blood stages and underlie the parasite's ability to establish chronic infection and transmit from human to mosquito. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the molecular epidemiology of the var multigene family at local sites in Gabon, Senegal and Kenya which differ in parasite prevalence and transmission intensity. 1839 distinct var gene types were defined by sequencing DBLα domains in the three sites. Only 76 (4.1%) var types were found in more than one population indicating spatial heterogeneity in var types across the African continent. The majority of var types appeared only once in the population sample. Non-parametric statistical estimators predict in each population at minimum five to seven thousand distinct var types. Similar diversity of var types was seen in sites with different parasite prevalences. CONCLUSIONS/SIGNIFICANCE: Var population genomics provides new insights into the epidemiology of P. falciparum in Africa where malaria has never been conquered. In particular, we have described the extensive reservoir of infection in local African sites and discovered a unique var population structure that can facilitate superinfection through minimal overlap in var repertoires among parasite genomes. Our findings show that var typing as a molecular surveillance system defines the extent of genetic complexity in the reservoir of infection to complement measures of malaria prevalence. The observed small scale spatial diversity of var genes suggests that var genetics could greatly inform current malaria mapping approaches and predict complex malaria population dynamics due to the import of var types to areas where no widespread pre-existing immunity in the population exists

The psychological science accelerator’s COVID-19 rapid-response dataset

In response to the COVID-19 pandemic, the Psychological Science Accelerator coordinated three large-scale psychological studies to examine the effects of loss-gain framing, cognitive reappraisals, and autonomy framing manipulations on behavioral intentions and affective measures. The data collected (April to October 2020) included specific measures for each experimental study, a general questionnaire examining health prevention behaviors and COVID-19 experience, geographical and cultural context characterization, and demographic information for each participant. Each participant started the study with the same general questions and then was randomized to complete either one longer experiment or two shorter experiments. Data were provided by 73,223 participants with varying completion rates. Participants completed the survey from 111 geopolitical regions in 44 unique languages/dialects. The anonymized dataset described here is provided in both raw and processed formats to facilitate re-use and further analyses. The dataset offers secondary analytic opportunities to explore coping, framing, and self-determination across a diverse, global sample obtained at the onset of the COVID-19 pandemic, which can be merged with other time-sampled or geographic data